abstract：:An endogenous inhibitor of neutral Ca2+-dependent proteinases has been isolated from rabbit liver cytosol. The inhibitor is a heat-stable, 240-kDa, tetrameric protein. It is dissociated into its 60-kDa subunits by high concentrations of Ca2+ (0.1-1 mM), but not by lower concentrations in the physiological range. Inhib...

journal_title：Archives of biochemistry and biophysics

authors： Melloni E,Salamino F,Sparatore B,Michetti M,Pontremoli S,Horecker BL

更新日期：1984-08-01 00:00:00

abstract：:During the deglycosylation reaction of fish egg polysialoglycoproteins under the conditions of 1 M NaBH4 in 0.1 M NaOH at 37 degrees C for 48 h, a marked loss of the glycine content has been encountered, besides the serine and threonine residues to which the carbohydrate units are linked. The chemical basis behind thi...

journal_title：Archives of biochemistry and biophysics

authors： Shimamura M,Inoue Y,Inoue S

更新日期：1984-08-01 00:00:00

abstract：:Superoxide generation, assessed as the rate of acetylated cytochrome c reduction inhibited by superoxide dismutase, by purified NADPH cytochrome P-450 reductase or intact rat liver microsomes was found to account for only a small fraction of their respective NADPH oxidase activities. DTPA-Fe3+ and EDTA-FE3+ greatly st...

journal_title：Archives of biochemistry and biophysics

authors： Morehouse LA,Thomas CE,Aust SD

更新日期：1984-07-01 00:00:00

abstract：:Total liver poly(A+)-RNA isolated from untreated, and 3-methylcholanthrene-, trans-stilbene oxide-, and phenobarbital-treated rats has been translated in the rabbit reticulocyte lysate system in order to determine the effect of these xenobiotics on the level of translationally active DT-diaphorase mRNA. The in vitro t...

journal_title：Archives of biochemistry and biophysics

authors： Williams JB,Wang R,Lu AY,Pickett CB

更新日期：1984-07-01 00:00:00

abstract：:The ability for various ligands to modulate the binding of fructose 1,6-bisphosphate (Fru-1,6-P2) with purified rat liver pyruvate kinase was examined. Binding of Fru-1,6-P2 with pyruvate kinase exhibits positive cooperativity, with maximum binding of 4 mol Fru-1,6-P2 per enzyme tetramer. The Hill coefficient (nH), an...

journal_title：Archives of biochemistry and biophysics

authors： Blair JB,Walker RG

更新日期：1984-07-01 00:00:00

abstract：:Infrared spectroscopy was used to investigate the changes in bands assigned to phospholipids and proteins in dehydrated and rehydrated sarcoplasmic reticulum. The changes in CH2 and CH3 stretching bands, amide bands, and phosphate stretching bands are similar to shifts in frequency seen for those bands in phospholipid...

journal_title：Archives of biochemistry and biophysics

authors： Crowe JH,Crowe LM,Chapman D

更新日期：1984-07-01 00:00:00

abstract：:Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

journal_title：Archives of biochemistry and biophysics

authors： Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

更新日期：1984-06-01 00:00:00

abstract：:Gossypol, a polyphenolic binaphthalene -dialdehyde reputed to exert contraceptive action in males, reversibly inhibits adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] in a concentration-dependent manner. In membranes prepared from a variety of organs, the half-maximal inhibitory concentration (IC50...

journal_title：Archives of biochemistry and biophysics

authors： Olgiati KL,Toscano DG,Atkins WM,Toscano WA Jr

更新日期：1984-06-01 00:00:00

abstract：:A new procedure for the purification of phospholipase C from Clostridium perfringens has been devised that results in essentially pure enzyme. The procedure consists of ammonium sulfate fractionation, ion-exchange chromatography on QAE-Sephadex, and affinity chromatography on phosphatidylcholine linked to Sepharose. T...

journal_title：Archives of biochemistry and biophysics

authors： Krug EL,Kent C

更新日期：1984-06-01 00:00:00

abstract：:The properties of porcine platelet acyltransferases which catalyze the incorporation of unsaturated fatty acids into the 2 positions of phospholipids were compared with those of porcine liver microsomes and rat liver microsomes. There were significant differences in the relative rates of incorporation of acyl groups i...

journal_title：Archives of biochemistry and biophysics

authors： Inoue M,Murase S,Okuyama H

更新日期：1984-05-15 00:00:00

abstract：:Rat hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was purified to homogeneity using agarose-HMG-CoA affinity chromatography. Additional protein was isolated from the affinity column with 0.5 M KCl that demonstrated no HMG-CoA reductase activity, yet comigrated with purified HMG-CoA reductase on sod...

journal_title：Archives of biochemistry and biophysics

authors： Whitehead TR,Vlahcevic ZR,Beg ZH,Hylemon PB

更新日期：1984-05-01 00:00:00

abstract：:delta 2-Isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase, which catalyzes the formation of isopentenyl-AMP from delta 2-isopentenylpyrophosphate and 5'AMP, was purified 6800-fold from the fruiting body of the cellular slime mold Dictyostelium discoideum using several separation procedures including 5'AMPo...

journal_title：Archives of biochemistry and biophysics

authors： Ihara M,Taya Y,Nishimura S,Tanaka Y

更新日期：1984-05-01 00:00:00

abstract：:The use of affinity chromatography on wheat germ agglutinin columns to partially purify detergent extracts of muscarinic acetylcholine receptor from porcine atria is described. The procedure results in a 20-fold purification of the protein. The partially purified protein binds [3H]L-QNB (the L isomer of quinuclidinyl ...

journal_title：Archives of biochemistry and biophysics

authors： Herron GS,Schimerlik MI

更新日期：1984-05-01 00:00:00

abstract：:Phosphatidylserine formation and decarboxylation are decreased in Tetrahymena in which phosphatidylethanolamine has been replaced by its isosteric analog 3-aminopropylphosphonolipid (1,2-diacylglyceryl-3-O-(3-aminopropylphosphonate). The combined activity of the phosphatidylethanolamine: serine phosphatidyltransferase...

journal_title：Archives of biochemistry and biophysics

authors： Smith JD

更新日期：1984-05-01 00:00:00

abstract：:The soluble ATPase isolated from Streptococcus faecalis membranes containing tightly bound endogenous nucleotides do not exchange in the presence of ATP and Mg+2 added during the purification of the enzyme. In this paper the stoichiometry of endogenous nucleotides in the soluble ATPase obtained from (a) growing cells,...

journal_title：Archives of biochemistry and biophysics

authors： Zlotnick GW,Abrams A

更新日期：1984-05-01 00:00:00

abstract：:High-Mr and monomeric adenosine deaminase were injected intravenously into the rabbit. The rates of clearance and sites of uptake of the enzymes were compared. Calf intestinal mucosa served as the source of monomeric adenosine deaminase. High-Mr enzymes were assembled in vitro from the calf enzyme and adenosine deamin...

journal_title：Archives of biochemistry and biophysics

authors： Schrader WP,Harder CM,Schrader DK,West CA

更新日期：1984-04-01 00:00:00

abstract：:The carbohydrate binding stoichiometry of lima bean lectin component III was reexamined using equilibrium dialysis and quantitative affinity chromatography following limited chemical modification. Equilibrium dialysis employing methyl[2-14C]benzamido-2-deoxy-alpha-D-galactopyranoside as ligand demonstrated that the le...

journal_title：Archives of biochemistry and biophysics

authors： Roberts DD,Goldstein IJ

更新日期：1984-04-01 00:00:00

abstract：:Rabbit anti-tyrosinase antibodies were used to study the abundance, processing, and degradation of tyrosinase in murine (Cloudman) melanoma cells. The polyclonal antibodies precipitated low-molecular-weight (68,000 and 70,000) and high-molecular-weight (78,000 and 80,000) tyrosinases that had a precursor-product relat...

journal_title：Archives of biochemistry and biophysics

authors： Halaban R,Pomerantz SH,Marshall S,Lerner AB

更新日期：1984-04-01 00:00:00

abstract：:The Mr 245,000 calmodulin-binding protein of the dogfish erythrocyte cytoskeleton (D245) has been compared with human erythrocyte spectrin and mammalian brain fodrin [J. Levine and M. Willard (1981) J. Cell Biol. 90, 631-643]. Mammalian erythrocyte alpha-spectrin, brain alpha-fodrin, and D245 are all localized in the ...

journal_title：Archives of biochemistry and biophysics

authors： Bartelt DC,Carlin RK,Scheele GA,Cohen WD

更新日期：1984-04-01 00:00:00

abstract：:An inhibitor of the transforming growth factor-induced growth in soft agar of normally anchorage-dependent rat kidney fibroblasts has been detected in the acid-ethanol extracts of human placenta, bovine lung and kidney, and human rhabdomyosarcoma cells (A673). The inhibitor has been purified from human placenta by gel...

journal_title：Archives of biochemistry and biophysics

authors： Frolik CA,Roller PP,Cone JL,Dart LL,Smith DM,Sporn MB

更新日期：1984-04-01 00:00:00

abstract：:If liver alcohol dehydrogenase were rate-limiting in ethanol metabolism, inhibitors of the enzyme should inhibit the metabolism with the same type of kinetics and the same kinetic constants in vitro and in vivo. Against varied concentrations of ethanol, 4-methylpyrazole is a competitive inhibitor of purified rat liver...

journal_title：Archives of biochemistry and biophysics

authors： Plapp BV,Leidal KG,Smith RK,Murch BP

更新日期：1984-04-01 00:00:00

abstract：:Studies were undertaken on the heat denaturation and proteolytic degradation by alpha-chymotrypsin of the normal red cell carbonic anhydrase isozyme, CA II, and two electrophoretic variants of carbonic anhydrase I, CA Ia and CA Ib, of the pigtail macaque. The heat degradation results showed a difference of about 40-fo...

journal_title：Archives of biochemistry and biophysics

authors： Osborne WR,Tashian RE

更新日期：1984-04-01 00:00:00

abstract：:The major phenobarbital-inducible form of cytochrome P-450 (cytochrome P-450 PB) was purified to homogeneity from rat liver microsomes and rabbit antibodies prepared against the purified enzyme. Using these antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed for the detection of cytochrome P-450 PB ...

journal_title：Archives of biochemistry and biophysics

authors： Seidel SL,Shawver LK,Shires TK

更新日期：1984-03-01 00:00:00

abstract：:Guanidine hydrochloride-induced denaturation and thermal denaturation of three kinds of tryptophan synthase alpha subunit have been compared by circular dichroism measurements. The three alpha subunits are from Escherichia coli, Salmonella typhimurium, and an interspecies hybrid in which the C-terminal domain comes fr...

journal_title：Archives of biochemistry and biophysics

authors： Yutani K,Sato T,Ogasahara K,Miles EW

更新日期：1984-03-01 00:00:00

abstract：:Calmodulin, a calcium-binding protein with no known enzymatic activity but multiple, in vitro effector activities, has been purified to apparent homogeneity from the unicellular green alga Chlamydomonas reinhardtii and compared to calmodulin from vertebrates and higher plants. Chlamydomonas calmodulin was characterize...

journal_title：Archives of biochemistry and biophysics

authors： Schleicher M,Lukas TJ,Watterson DM

更新日期：1984-02-15 00:00:00

abstract：:The hydrolysis of 32 X-phenyl-N-methanesulfonyl glycinates by papain was investigated. It was found that the variation in the Michaelis constants could be rationalized by the following correlation equation: log 1/Km = 0.61 pi '3 + 0.46 MR4 + 0.55 sigma + 2.00 with a correlation coefficient of 0.945. In this expression...

journal_title：Archives of biochemistry and biophysics

authors： Carotti A,Smith RN,Wong S,Hansch C,Blaney JM,Langridge R

更新日期：1984-02-15 00:00:00

abstract：:Periodate-oxidized NADP+ (dialdehyde-NADP+) inactivated soluble ferredoxin-NADP+ oxidoreductase and combined covalently to the enzyme. This inactivation was first order with respect to dialdehyde-NADP+ and followed saturation kinetics, indicating that the enzyme initially forms a reversible complex with the inactivato...

journal_title：Archives of biochemistry and biophysics

authors： Chan RL,Carrillo N

更新日期：1984-02-15 00:00:00

abstract：:The in vivo binding of platinum to metallothionein (MT) has been observed in rat tissues following injections of the cis and trans isomers of DDP (dichlorodiammineplatinum(II]. Platinum in either cis-DDP or trans-DDP does not directly induce MT; platinum-MT is produced by the replacement of previously bound zinc in th...

journal_title：Archives of biochemistry and biophysics

authors： Zelazowski AJ,Garvey JS,Hoeschele JD

更新日期：1984-02-15 00:00:00

abstract：:Proteolysis of glucose dehydrogenase from Bacillus megaterium with proteinase K apparently generated two fragments. The small fragment, designated as K-peptide, was sequenced and its covalent structure was determined as Ser-Ser-Glu-Ala-Ser-Tyr-Val-Thr-Gly-Ile-Thr-Leu-Phe-Ala-Asp-Gly-Gly-Met-Thr- Gln-Tyr-Pro-Ser-Phe-Gl...

journal_title：Archives of biochemistry and biophysics

authors： Jany KD,Nitsche E

更新日期：1984-02-15 00:00:00

abstract：:The stoichiometry of complex formation between two lysosomal proteinases from rabbit liver, cathepsin M and fructose 1,6-bisphosphatase converting enzyme (CE), and their respective endogenous inhibitors was studied by the equilibrium gel penetration method. In each case the molecular weight of the complex was found to...

journal_title：Archives of biochemistry and biophysics

authors： Pontremoli S,Melloni E,Salamino F,Sparatore B,Michetti M,Horecker BL

更新日期：1984-02-01 00:00:00

abstract：:Rat bone was extracted with KCl and Triton X-100, and a tartrate-resistant acid phosphatase activity was purified by protamine sulfate precipitation, ion-exchange chromatography (CM-cellulose), and gel filtration on Sephadex G-200 according to previously described procedures. Sodium dodecyl sulfate-polyacrylamide gel ...

journal_title：Archives of biochemistry and biophysics

authors： Andersson G,Ek-Rylander B,Hammarström L

更新日期：1984-02-01 00:00:00

abstract：:Dopamine beta-hydroxylase present in the soluble matrix of bovine adrenal medullary chromaffin granules contains biantennary complex oligosaccharides and high-mannose oligosaccharides in a molar ratio of approximately 2:1. The high-mannose oligosaccharides contain an average of six mannose residues. The largest biante...

journal_title：Archives of biochemistry and biophysics

authors： Margolis RK,Finne J,Krusius T,Margolis RU

更新日期：1984-02-01 00:00:00

abstract：:Studies of modification of hemoglobin and of sickle hemoglobin by alternative aspirins have been extended to a series of new bis esters with a variety of substituted bridging diacids and to a group of mono esters with polar acyl groups. Rates of hydrolysis of these alternative aspirins have also been examined, and the...

journal_title：Archives of biochemistry and biophysics

authors： Delaney EJ,Massil SE,Shi GY,Klotz IM

更新日期：1984-02-01 00:00:00

abstract：:The topological arrangement of cytochrome b561 in the bovine adrenal medullary chromaffin granule membrane was investigated by radiolabeling and immunoprecipitation techniques using antibody raised against the purified cytochrome. The first labeling procedure involved a membrane-permeable amino group labeling reagent,...

journal_title：Archives of biochemistry and biophysics

authors： Duong LT,Fleming PJ

更新日期：1984-01-01 00:00:00

abstract：:The effects of the addition of hypolipidemic drugs and 1-acylglycerolipids on the metabolism of oleate in plants have been studied in vivo and in vitro. Using aged potato slices with [14C]oleate as a precursor, it was found that these drugs markedly inhibited both the incorporation into complex lipids and the desatura...

journal_title：Archives of biochemistry and biophysics

authors： Sánchez J,Stumpf PK

更新日期：1984-01-01 00:00:00

abstract：:A chemical investigation has been done on blood group active glycosphingolipids of both small intestine and pancreas from two individuals, one blood group A and one blood group B. Total non-acid glycolipid fractions were prepared and the major blood group fucolipids present were purified and structurally characterized...

journal_title：Archives of biochemistry and biophysics

authors： Breimer ME

更新日期：1984-01-01 00:00:00

abstract：:Sixteen mutants of Escherichia coli defective in H+-ATPase (proton-translocating ATPase) were tested for their ability to recombine with hybrid plasmids carrying various portions of the beta subunit cistron. Twelve mutations were mapped within the carboxyl half of the cistron corresponding to amino acid residues 279 t...

journal_title：Archives of biochemistry and biophysics

authors： Kanazawa H,Noumi T,Oka N,Futai M

更新日期：1983-12-01 00:00:00

abstract：:Binding sites having the properties of high-affinity receptors for activated alpha 2-macroglobulin (alpha 2M) have been purified over 100-fold from membranes of spontaneously transformed NIH-3T3 cells (J. A. Hanover, S.-y. Cheng, M. C. Willingham, and I. H. Pastan [1983] J. Biol. Chem. 258, 370-377). To identify the m...

journal_title：Archives of biochemistry and biophysics

authors： Hanover JA,Rudick JE,Willingham MC,Pastan I

更新日期：1983-12-01 00:00:00

abstract：:Heparin is thought to regulate the rate of mammalian blood clotting by enhancing the activity of antithrombin, an inhibitor of coagulation enzymes. The present study establishes that this same inhibitor is present in the blood plasma of each of the terrestrial vertebrate groups including mammals, birds, reptiles, and ...

journal_title：Archives of biochemistry and biophysics

authors： Jordan RE

更新日期：1983-12-01 00:00:00

abstract：:The soluble form of mitochondrial adenosine triphosphatase was purified in an electrophoretically and immunologically pure form from sweet potato root tissue. The enzyme consisted of six kinds of subunits with different molecular weights (52,500, 51,500, 35,500, 26,000, 23,000, and 12,000), and its molecular weight wa...

journal_title：Archives of biochemistry and biophysics

authors： Iwasaki Y,Asahi T

更新日期：1983-11-01 00:00:00